Manual of Methods of Pure Culture Study
ISBN : 9789385961069
Author(s) : Abhay B. Solunke, Venkat S. Hamde, Prashant S. Wakte, Rajendra S. Awasthi
Year : 2015
Edition : 1
Cover Type : Hard Cover
Size : 9.5 x 6.5
Weight : 610 gr./327P
Publisher : NPI
Subject : Microbiology
Language : English
Price : INR.1360 /- Per Unit (soft copy also available)
Price : USD.136 /- Per Unit (soft copy also available)
Discount : Rs.340 /- Per Unit
Shipping : Rs.65 /- Per Unit
Dr. Abhay B. Solunke
Head Department of Microbiology,
Shri Govindrao Munghate Arts& Science College,
Kurkheda.441 209
Dist. Gadchiroli (Maharashtra)
(Dr. Abhay B. Solunke did his M.Sc. from Marathwada University Aurangabad in 1993. He is recognised University PG teachers in Microbiology and Biotechnology of Swami Ramanand Teerth Marathwada University, Nanded and has taught devotedly at undergraduate and postgraduate level. He has several research papers and projects to his credit and also authored books in his field of study. He has introduced M.Sc. Industrial Microbiology as innovative subject in UGC X plan programme. His Ph.D. work is associated with Mushrooms and the bioconversion of agro-wastes. He is also Ph.D. Guide in Microbiology and Biotechnology of Gondwana University, Gadchiroli. He is also Chairman of Board of studies in Microbiology and Biotechnology and Member of Academic Council Gondwana University, Gadchiroli. He is currently serving as Head Department of Microbiology and IQAC coordinator at Shri Govindrao Munghate Arts & Science College, Kurkheda. Dist. Gadchiroli. )
Dr. Venkat S. Hamde
Department of Microbiology,
Yogeshwari Mahavidyalaya,Ambajogai
Dist. Beed(Maharashtra)
Dr. Prashant S. Wakte
Head Department of Microbiology,
DSM College, Parbhani. 431 401(MS)
Dr. Rajedra S. Awasthi
Head Department of Microbiology,
Shivaji College, Renapur. 413 527(Maharashtrs)
This manual is intended for use in those types of microbiological work which involve the study of microbial cultures or of viruses, either for identification or for learning the properties of the organisms investigated.
This Manual is intended for use in that type of microbiological work known as "pure culture study of bacteria".
This book takes the place of the loose-leaf publication issued during the period of 1923-2013 under the name of ''Manual of Methods For Pure Culture Study." The present manual covers a wider scope.
The standardization of methods tends to hinder the development of new techniques, while the chief function of this manual is to stimulate its development.
The methods in this manual, therefore, are merely claimed to be those regarded as satisfactory.
A thorough course of laboratory instruction in Microbiology is absolutely essential to the proper education of the student of the present day. The practical knowledge thus acquired in the methods of handling bacteria, in the precautions necessary to the prevention of personal infection, and in the methods for the recognition and for the destruction of disease-producing organisms is fundamental and invaluable. Such information is directly useful
as a means of diagnosis; it is necessary to the successful performance of antiseptic operations and is indispensable to the proper execution and understanding of the common hygienic measures for the prevention of communicable diseases.
It is therefore evident that the course in Microbiology should not be inferior, either in length or in the character of the instruction, to any other laboratory course offered in the Microbiology curriculum. The student should be taught to work, not merely with a few harmless bacteria, but especially with all of the common pathogenic organisms. The exclusion of the latter organisms from a laboratory course on the plea of danger is an admission of weakness in instruction or in supervision.
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Sr. No. |
Title |
Page No. |
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1 |
Media and Cultural Techniques |
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1.1 |
Introduction to Culture Media, Stains and Solutions. |
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1.2 |
Preparation of Culture Media |
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1.3 |
Discovery of Agar |
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1.4 |
MacConkey agar |
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1.5 |
Cotton Plug |
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1.6 |
Petri Plates |
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1.7 |
Reinforced Clostridial Medium |
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1.8 |
Agarose |
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1.9 |
Beef Extract |
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1.10 |
Malt Extract |
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1.11 |
Peptone |
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1.12 |
Meat Peptones |
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1.13 |
Casein Peptones |
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1.14 |
Soy Peptones |
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1.15 |
Casein Hydrolysate |
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1.16 |
Lactoalbumin Hydrolysate |
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1.17 |
Malt Extract |
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1.18 |
Mycological Peptone |
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1.19 |
Peptonized Milk |
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1.20 |
Proteose Peptone |
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1.21 |
Sheep Blood, Defibrinated |
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1.22 |
Tryptone |
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1.23 |
Tryptose |
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1.24 |
Yeast Extract |
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1.25 |
Biphasic media |
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1.26 |
Streak Plate Method |
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1.27 |
Autoclaving |
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1.28 |
pH Indicators |
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1.29 |
Water Activity |
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1.30 |
Dyes for Culture Media |
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1.31 |
Different Categories of Culture Media |
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1.32 |
Media and Purpose |
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1.33 |
Storage of culture media |
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1.34 |
Quantal microbiology |
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1.35 |
Bacterial Colony Characteristics |
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1.36 |
Meaning of pure culture study |
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1.37 |
History of Methods for Pure Culture Study |
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1.38 |
Determining the characteristics of a culture |
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1.39 |
Relation to Taxonomy |
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1.40 |
Media used in pure culture study |
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1.41 |
Natural storage media |
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1.42 |
Indicator media |
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1.43 |
Use of Methylene Blue as Indicator of Anaerobiosis |
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1.44 |
Use of aerobe to absorb oxygen |
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1.45 |
Microscopic Examination of Pure Cultures |
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1.46 |
The Use of Serology In Pure Culture Study |
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1.47 |
Maintenance and Preservation of Cultures |
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1.48 |
Selection of Materials |
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1.49 |
Cleaning Glassware’s |
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1.50 |
Preparation of Glassware For Sterilization |
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1.51 |
Sterilization |
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1.52 |
Titration of Media |
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1.53 |
Fuller's Scale |
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1.54 |
Platinum needles |
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1.55 |
Inoculating Tube Cultures |
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1.56 |
Caoutchouc Caps |
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1.57 |
Serum Inspissator |
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1.58 |
Old Apparatus for Storing and for Cultivations in Liquid Media |
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1.59 |
The Growth of the Microbial Cell in Pure Culture and in Mixed Populations |
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1.60 |
Principles of Microbial Nutrition and Composition of Culture Media for Microorganisms |
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1.61 |
Cotton Filters |
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SECTION –I: CULTURE MEDIA |
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1 |
A1 Medium |
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2 |
A3 Agar |
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3 |
A7 Agar |
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4 |
ABY Agar (Acid Bismuth Yeast Agar) |
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5 |
Abeyta-Hunt Bark Agar |
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6 |
Actidione (Cycloheximide) Agar |
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7 |
Acetamide Agar |
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8 |
Acetamide Broth |
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9 |
Acetate Agar |
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10 |
Acetate differential |
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11 |
Acetivibrio celluloyticus Medium |
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12 |
Actinomyces Broth/Agar |
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13 |
Actinomycete Isolation Agar with Glycerol |
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14 |
Acetobacter diazotrophicus Agar |
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15 |
Acetobacterium dehalogenans Medium |
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16 |
Acetogen Medium |
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17 |
Acetohalobium Medium |
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18 |
Acetylglucosamine Medium |
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|
19 |
Acid Glucose Salts Medium |
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20 |
Acid Rhodospirillaceae Medium |
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|
21 |
Acid Egg Medium |
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22 |
Acid Tomato Broth |
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23 |
Acidic Tomato Medium for Leuconostoc |
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|
24 |
Actinomyces Broth |
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|
25 |
Actinomycete Growth Medium |
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26 |
Acidobacterium Medium |
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27 |
AE Sporulation Medium, Modified |
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|
28 |
Aeromonas Differential Agar |
|
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29 |
Aeromonas Medium |
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30 |
AFPA (Aspergillus flavus/parasiticus Agar) |
|
|
31 |
Agrobacterium Agar |
|
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32 |
AK Agar |
|
|
33 |
AKI Medium |
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|
34 |
Alcaligenes Agar |
|
|
35 |
Alginate Utilization Medium |
|
|
36 |
Alkaline Cellulose Agar |
|
|
37 |
Alkaline Nutrient Agar |
|
|
38 |
Alkaline Peptone Agar |
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|
39 |
Alkaline Peptone Water |
|
|
40 |
Alkaline Polypectate Agar |
|
|
41 |
Alkaline Starch Agar |
|
|
42 |
Alkaliphilic Methanogen Medium |
|
|
43 |
Alkaliphilic Spirochete Medium |
|
|
44 |
Alkalophile Medium |
|
|
45 |
Alkalophilic Halophile Agar |
|
|
46 |
Allantoin Agar |
|
|
47 |
ALP Basal Medium |
|
|
48 |
Amino-butyric Acid Medium |
|
|
49 |
Amies Transport Medium With Charcoal |
|
|
50 |
Amies Transport Medium Without Charcoal |
|
|
51 |
Amino Acid Assay Media. Lysine Assay Medium • Cystine Assay Medium |
|
|
52 |
AMS Agar |
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|
53 |
Ammonium Agar |
|
|
54 |
Amygdalin Medium |
|
|
55 |
Anaerobic Agar |
|
|
56 |
Andrade’s Carbohydrate Broth and Indicator |
|
|
57 |
Antibiotic Medium (Seed Agar) |
|
|
58 |
Antibiotic Medium (Base Agar) |
|
|
59 |
Antibiotic Medium |
|
|
60 |
Antibiotic Medium (For Streptomycin Assays) |
|
|
61 |
Antibiotic Medium (Base Agar With Low pH) |
|
|
62 |
Anaerobic Agar |
|
|
63 |
Anaerobic Cellulolytic Medium |
|
|
64 |
AOAC Letheen Broth |
|
|
65 |
AO Agar |
|
|
66 |
Apple Juice Yeast Extract Medium |
|
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67 |
APT Agar/APT Broth |
|
|
68 |
Arginine Broth |
|
|
69 |
Armstrong Fusarium Medium |
|
|
70 |
Artificial Deep Lake Medium |
|
|
71 |
Artificial Organic Lake Peptone Medium |
|
|
72 |
Arylsulfatase Agar |
|
|
73 |
Ascospore Agar |
|
|
74 |
Ashbey’s Nitrogen-Free Agar |
|
|
75 |
Asparagine Broth |
|
|
76 |
Asparagine Broth |
|
|
77 |
Aureomycin Rose Bengal Glucose Peptone Agar |
|
|
78 |
Autotrophic Nitrobacter Medium |
|
|
79 |
Azide Blood Agar |
|
|
80 |
Azide Blood Agar with Crystal Violet |
|
|
81 |
Azide Broth |
|
|
82 |
Azide Citrate Broth |
|
|
83 |
Azospirillum Medium |
|
|
84 |
Azotobacter Agar |
|
|
85 |
Azotobacter Broth |
|
|
86 |
Azotobacter chroococcum Agar |
|
|
87 |
Azotobacter paspali Medium |
|
|
88 |
Azotobacter vinelandii Medium |
|
|
89 |
Baar’s Medium for Sulfate Reducers |
|
|
90 |
Bacillus Agar |
|
|
91 |
Bacillus halodenitrificans Agar |
|
|
92 |
Bacillus Medium |
|
|
93 |
Bacillus Medium |
|
|
94 |
Selective Agar Base For Bacillus Cereus. |
|
|
95 |
Bacillus thuringiensis Medium |
|
|
96 |
BCP Azide Broth |
|
|
97 |
Bdellovibrio Medium |
|
|
98 |
Beef Extract Agar |
|
|
99 |
Beef Extract Broth |
|
|
100 |
Beggiatoa and Thiothrix Medium |
|
|
101 |
Beijerinckia Medium |
|
|
102 |
Beijerinck’s Thiobacillus Medium |
|
|
103 |
Benzoate Medium |
|
|
104 |
Bile Salts Gelatin Agar |
|
|
105 |
Biphenyl Agar |
|
|
106 |
Bismuth Sulfite Broth |
|
|
107 |
Blood Agar |
|
|
108 |
Blood Agar Base |
|
|
109 |
Blood Agar With Nalidixic Acid Base |
|
|
110 |
Blood Agar Base with 2.5% NaCl |
|
|
111 |
Blood Glucose Cystine Agar |
|
|
112 |
Blue-Green Nitrogen-Fixing Agar |
|
|
113 |
Brackish Acetate |
|
|
114 |
Brevibacterium Medium |
|
|
115 |
Brilliant Green Bile Agar |
|
|
116 |
Brilliant Green Bile Broth |
|
|
117 |
Brilliant Green Bile Broth with MUG |
|
|
118 |
Brilliant Green Broth |
|
|
119 |
Bromcresol Purple Broth |
|
|
120 |
Bromcresol Purple Dextrose Broth |
|
|
121 |
Bromothymol Blue Agar |
|
|
122 |
Bryant- Burkey Broth |
|
|
123 |
Burke’s Modified Nitrogen-Free Medium |
|
|
124 |
Brucella Broth |
|
|
125 |
Bushnell-Haas Agar |
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|
126 |
Butanediol Medium |
|
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127 |
Camphor Minimal Medium |
|
|
128 |
Carbohydrate Fermentation Broth |
|
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129 |
Carbon Monoxide Oxidizers Agar, Modified |
|
|
130 |
Carbon Utilization Test |
|
|
131 |
Carboxydobacterium Medium |
|
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132 |
Casein Medium |
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133 |
Caulobacter Medium |
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134 |
Cellulolytic Agar for Thermophiles |
|
|
135 |
Cellulolytic Broth with Sea Salts |
|
|
136 |
Cellulolytic Medium |
|
|
137 |
Cellulolytic Medium with Rumen Fluid and Soluble Starch |
|
|
138 |
Cellulomonas fermentans Medium |
|
|
139 |
Cellulose Anaerobe Medium |
|
|
140 |
Cellulose Broth |
|
|
141 |
Cellvibrio Medium |
|
|
142 |
Centenum Medium |
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|
143 |
Cetrimide Agar |
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|
144 |
Chitin Agar |
|
|
145 |
Chitin Agar |
|
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146 |
Chlorobutane Medium |
|
|
147 |
Chlorohydroxybenzoic Acid Medium |
|
|
148 |
Cholera Medium TCBS |
|
|
149 |
Citrate Medium, Koser’s Modified |
|
|
150 |
Citrate Phosphate Buffered Glucose Medium |
|
|
151 |
Clostridium beijerinckii Agar |
|
|
152 |
Clostridium Cellulolytic Medium |
|
|
153 |
Clostridium Cellulose Medium |
|
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154 |
Clostridium kluyveri Agar |
|
|
155 |
Clostridium pfennigii Medium |
|
|
156 |
Clostridium propionicum Medium |
|
|
157 |
Clostridium thermohydrosulfuricum Medium |
|
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158 |
Colby and Zatman Agar |
|
|
159 |
Colloidal Chitin Agar |
|
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160 |
Cook’s Cytophaga Agar |
|
|
161 |
Cornmeal Agar(CMA) |
|
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162 |
Cornmeal Agar with Soil Extract |
|
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163 |
Cornmeal Yeast Extract Seawater Agar |
|
|
164 |
Corynebacterium Medium with Salt |
|
|
165 |
Cow Manure Agar |
|
|
166 |
CP Medium for Coprothermobacter proteolyticus |
|
|
167 |
Cyclohexanone Medium |
|
|
168 |
Czapek Agar |
|
|
169 |
Czapek Dox Agar, Modified |
|
|
170 |
Czapek Dox Broth |
|
|
171 |
Czapek Solution Agar with Sucrose |
|
|
172 |
Deoxycholate Agar |
|
|
173 |
Deoxycholate Citrate Agar |
|
|
174 |
Deoxyribonuclease (DNase Test) Agar (pH 7.3) |
|
|
175 |
Desulfovibrio Medium |
|
|
176 |
Dextrose Broth |
|
|
177 |
Dextrose Soil Agar(DSA) |
|
|
178 |
Diazotrophic Medium(RBA) |
|
|
179 |
Dibenzothiophene Mineral Medium |
|
|
180 |
Dichloroacetic Acid Medium |
|
|
181 |
Dichloromethane Medium for Hyphomicrobium |
|
|
182 |
DNase Medium |
|
|
183 |
Dubos Agar with Filter Paper |
|
|
184 |
Dubos Mineral Medium |
|
|
185 |
Dubos Salts Agar |
|
|
186 |
Dubos Salts Broth with Yeast Extract |
|
|
187 |
E Agar(m-E Agar) |
|
|
188 |
EC Broth(Escherichia coli Broth)(EC Medium) |
|
|
189 |
EC Broth with MUG |
|
|
190 |
EIA Substrate |
|
|
191 |
Eijkman Lactose Medium |
|
|
192 |
EMB Agar(Eosin Methylene Blue Agar) |
|
|
193 |
EMB Agar, Modified(Eosin Methylene Blue Agar, Modified) |
|
|
194 |
Emerson Agar |
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|
195 |
Endo Agar |
|
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196 |
Endo Agar, LES(Endo Agar, Laurance Experimental Station) (m-Endo Agar, LES)(m-LES, Endo Agar) |
|
|
197 |
Endo Broth(m-Endo Broth) |
|
|
198 |
Enriched Cytophaga Agar |
|
|
199 |
Enterococci Confirmatory Agar |
|
|
200 |
Enterococci Presumptive Broth |
|
|
201 |
Enterococcus Agar(m-Enterococcus Agar)(Azide Agar) |
|
|
202 |
Erwinia Fermentation Medium |
|
|
203 |
Esculin Agar |
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|
204 |
Esculin Iron Agar |
|
|
205 |
Ethyl Violet Azide Broth(EVA Broth) |
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206 |
Ethyl Violet Azide Broth(EVA Broth) |
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|
207 |
Ethyl Violet Azide Broth(EVA Broth) |
|
|
208 |
Extracted Hay Medium |
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|
209 |
EYGA Agar |
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|
210 |
Fay and Barry Medium |
|
|
211 |
FC Agar (Fecal Coliform Agar)(m-FC Agar) (m-Fecal Coliform Agar) |
|
|
212 |
Fecal Coliform Agar, Modified(m-Fecal Coliform Agar, Modified)(FCIC) |
|
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213 |
Fecal Coliform Agar, Modified |
|
|
214 |
Feodorov Medium |
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|
215 |
FGTC Agar |
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|
216 |
Fish Peptone Broth |
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|
217 |
Flavobacterium Medium |
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|
218 |
Fletcher Medium |
|
|
219 |
Flo Agar |
|
|
220 |
Fluorescent Pectolytic Agar(FPA Medium) |
|
|
221 |
FN Medium(Fluorescence Denitrification Medium) |
|
|
222 |
FPA Medium |
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|
223 |
Frankia Agar |
|
|
224 |
Fraser Broth |
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|
225 |
Freshwater Ameba Medium |
|
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226 |
FSM Selective Medium |
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|
227 |
FUF Medium |
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|
228 |
Gelatin Agar |
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|
229 |
General Salts Medium for Estuarine Methanogens |
|
|
230 |
Geo Medium |
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231 |
George’s Medium, Modified |
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|
232 |
Gisa Agar |
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233 |
Gluconate Peptone Broth |
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234 |
Glucose Agar, 9K |
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|
235 |
Glucose Agar with 25%Glucose |
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|
236 |
Glucose Nitrogen-Free Salt Agar |
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|
237 |
Glucose Peptone Agar |
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|
238 |
Glucose Yeast Chalk Agar |
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|
239 |
Glucose Yeast Extract Agar |
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|
240 |
Glutamate Medium |
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|
241 |
Glycerol Agar |
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|
242 |
Glycerol Arginine Agar |
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|
243 |
Glycerol Chalk Agar |
|
|
244 |
Gorham’s Medium for Algae |
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245 |
Hagedorn and Holt Selective Medium |
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|
246 |
Haloalkaliphilic Agar |
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|
247 |
Halobacteriaceae Medium |
|
|
248 |
Halobacterium halobium Defined Medium |
|
|
249 |
Halophile Agar |
|
|
250 |
Halophile Medium |
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|
251 |
Halophilic Broth(HB) |
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|
252 |
Halophilic Nutrient Agar |
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253 |
Halophilic Synthetic Medium |
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254 |
Hartley’s Digest Broth |
|
|
255 |
Hay Infusion Agar |
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|
256 |
HE Medium(Hay Extract Medium) |
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|
257 |
Heart Infusion Agar |
|
|
258 |
Hektoen Enteric Agar |
|
|
259 |
Heterotrophic Hyperthermophilic Archaea Medium |
|
|
260 |
Heterotrophic Medium H3P |
|
|
261 |
Heterotrophic Medium for Hydrogenomonas |
|
|
262 |
Heterotrophic Medium for Hydrogen-Oxidizing Bacteria |
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|
263 |
Heterotrophic Nitrobacter Medium |
|
|
264 |
HPC Agar (Heterotrophic Plate Count Agar) (m-HPC Agar) |
|
|
265 |
Hugh-Leifson’s Glucose Broth |
|
|
266 |
Modified Hutner’s Basal Salts |
|
|
267 |
Hydrogen-Oxidizing Bacteria Medium |
|
|
268 |
Hydroxybenzoate Agar(p-Hydroxybenzoate Agar) |
|
|
269 |
Hyphomicrobium Enrichment Medium |
|
|
270 |
Imhoff’s Medium, Modified |
|
|
271 |
Imidazole Utilization Medium |
|
|
272 |
Inorganic Salts-Maltose Medium |
|
|
273 |
Inositol Brilliant Green Bile Salts Agar(IBB Agar) |
|
|
274 |
Iron Bacteria Isolation Medium |
|
|
275 |
Iron-Oxidizing Medium |
|
|
276 |
Iron Sulfite Agar |
|
|
277 |
Isoleucine Hydroxamate Medium |
|
|
278 |
Johnson’s Marine Medium |
|
|
279 |
Kerosene Mineral Salts Medium |
|
|
280 |
Ketolactonate Broth |
|
|
281 |
Kligler Iron Agar (pH 7.4) |
|
|
282 |
Kligler Iron Agar |
|
|
283 |
King’s Medium |
|
|
284 |
Kleb Agar(m-Kleb Agar) |
|
|
285 |
Koser Citrate Medium |
|
|
286 |
KYE Agar |
|
|
287 |
Lactate Agar |
|
|
288 |
Lactose Broth |
|
|
289 |
Lactose Minimal Medium |
|
|
290 |
Lactose Ricinoleate Broth |
|
|
291 |
Lauryl Sulfate Broth(m-Lauryl Sulfate Broth) |
|
|
292 |
Lauryl Sulfate Broth(Lauryl Tryptose Broth) |
|
|
293 |
Lauryl Sulfate Broth with MUG |
|
|
294 |
Lauryl Tryptose Mannitol Broth with Tryptophan |
|
|
295 |
LB Agar |
|
|
296 |
Lead Acetate Agar |
|
|
297 |
Legume Extract Agar |
|
|
298 |
Leptospira Medium |
|
|
299 |
Leuconostoc Medium |
|
|
300 |
Leucothrix mucor Medium |
|
|
301 |
Levine EMB Agar(Levine Eosin Methylene Blue Agar) (Eosin Methylene Blue Agar, Levine)(LEMB Agar) |
|
|
302 |
Lima Bean Agar |
|
|
303 |
Lipovitellin Salt Mannitol Agar |
|
|
304 |
Litmus Milk |
|
|
305 |
Lysine Decarboxylase Broth, Falkow |
|
|
306 |
Lysine Decarboxylase Broth, Taylor Modification |
|
|
307 |
Lysine Decarboxylase Medium |
|
|
308 |
Lysine Iron Agar |
|
|
309 |
MacConkey Agar |
|
|
310 |
MacConkey Broth |
|
|
311 |
MacConkey Broth, Purple |
|
|
312 |
Malachite Green Broth |
|
|
313 |
Malonate Broth |
|
|
314 |
Malt Agar |
|
|
315 |
Manganese Acetate Agar |
|
|
316 |
Manganese Medium for Pseudomonas species |
|
|
317 |
Mannitol Yeast Extract Medium |
|
|
318 |
Marine Agar |
|
|
319 |
Marine Agar with Biphenyl |
|
|
320 |
Marine Agar with Naphthalene |
|
|
321 |
Marine Flagellate Medium |
|
|
322 |
Marine Rhodococcus Medium |
|
|
323 |
Marine Rhodopseudomonas Medium |
|
|
324 |
Medium for Ammonia Oxidizers, Marine |
|
|
325 |
Medium for Carbon Monoxide Oxidizers |
|
|
326 |
Medium F for Sulfate Reducers(Postgate’s Medium F for Sulfate Reducers) |
|
|
327 |
Medium for Halophilic Bacilli |
|
|
328 |
Medium for Hydrocarbon-Degrading Bacteria |
|
|
329 |
Medium for Hydrocarbon-Degrading Bacteria (Naphthalene Mineral Salts Medium) |
|
|
330 |
Medium for Nitrite Oxidizers |
|
|
331 |
Medium for Nitrite Oxidizers, Marine |
|
|
332 |
Medium for Osmophilic Fungi |
|
|
333 |
Medium for Thermophilic Actinomycetes |
|
|
334 |
Membrane Lauryl Sulfate Broth |
|
|
335 |
Methanobacillus Medium |
|
|
336 |
Methanobacteria Medium |
|
|
337 |
Methanobacterium thermoautotrophicum MS Medium |
|
|
338 |
Methanogen Enrichment Medium, Barker |
|
|
339 |
Methanogen Medium |
|
|
340 |
Methanol Agar |
|
|
341 |
Methylamine Salts Medium |
|
|
342 |
Mineral Medium with 2,4- Dichlorophenoxy acetic Acid |
|
|
343 |
Mineral Medium, Nagel and Andreesen |
|
|
344 |
Mineral Medium with Naphthalene |
|
|
345 |
Mineral Medium with Phenol |
|
|
346 |
Mineral Medium with Pyrrolic Acid |
|
|
347 |
Mineral Medium with Salicylate |
|
|
348 |
Mineral Medium with Toluene |
|
|
349 |
Moller Decarboxylase Broth |
|
|
350 |
Moller KCN Broth Base |
|
|
351 |
Motility Test Media (pH 7.2) |
|
|
352 |
MPH Agar(Milk Protein Hydrolysate Agar) |
|
|
353 |
MPSS Broth |
|
|
354 |
MRVP Broth(Methyl Red Voges-Proskauer Broth) |
|
|
355 |
Mycorrhiza Medium |
|
|
356 |
Myxobacteria Medium |
|
|
357 |
Nine K Medium (9K Medium) |
|
|
358 |
Nitrate Agar |
|
|
359 |
Nitrate Reduction Broth for Pseudomonas and Related Genera |
|
|
360 |
Nitrogen-Fixing Hydrocarbon Oxidizers Medium |
|
|
361 |
Nitrogen-Fixing Marine Medium |
|
|
362 |
Nitrogen-Free Agar |
|
|
363 |
Nitrogen-Free Agar (Norris Agar) |
|
|
364 |
Nitrosomonas Medium |
|
|
365 |
Nutrient Agar |
|
|
366 |
Nutrient Agar pH 10.0 |
|
|
367 |
Nutrient Soil Extract Agar |
|
|
368 |
NWRI Agar(HPC Agar) |
|
|
369 |
Oat Flake Medium |
|
|
370 |
Oil Agar Medium |
|
|
371 |
Oxidation-Fermentation Medium(OF Medium) |
|
|
372 |
Oxidation-Fermentation, Medium, King’s (King’s OF Medium) |
|
|
373 |
PA-C Agar(mPA-C Agar) |
|
|
374 |
Paraffin Agar |
|
|
375 |
Payne, Seghal, and Gibbons Medium |
|