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NPI International Research Journal of Industrial and Environmental Biotechnology(Volume-1) Issue-1&2

ISSN : 2581-7906

Frequency : Bi-Annual

Publisher : NPI

Category : Biotchnology, Environmental, Environmental

Language : English

Price : INR.4000 /- Per Subscription (soft copy also available)

Price : USD.100 /- Per Subscription (soft copy also available)

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Prof. Lala Behari Sukla
Multi-Disciplinary Research Cell, Siksha ‘O’ Anusandhan University, Bhubaneswar-751030, India

Editorial Board Members

Prof. Irena Twardowska
PhD., DSc., Polish Academy of Sciences, Poland

Prof. Pattanathu K.S.M. Rahman
Technology Futures Institute, School of Science and Engineering, Teesside University, Middlesbrough, UK

Prof. Roberto Acevedo

Universidad de Chile, Santiago, CHILE

Dr. E. Subudhi
CBT, Siksha ‘O’ Anusandhan University, Bhubaneswar, India

Dr. Sradhanjali Singh,
NEERI Zonal Laboratory, New Delhi

Prof. G.K. Roy
Ex-Director, National Institute of Technology, Rourkela, India

Prof. Ata Akcil
Ph.D. Eng., Suleyman Demirel University, Engineering Faculty, Isparta, Turkey

Dr. N. Pradhan
Sr. Scientist, CSIR-IMMT, Bhubaneswar

Dr. Debabrata Pradhan
BBRC, Siksha ‘O’ Anusandhan University, Bhubaneswar, India

Dr. Sandeep Panda
Suleyman Demirel University, Isparta, Turkey

Er. Bibhudhendu Shukla
Data Scientist, UK

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Bioremediation of Water: Biosorption of Co(II) on Cinamomum zy. Leaf Powder

Sridhara Acharya, CSIR-IMMT (Formerly RRL), Bhubaneswar 751 013, Odisha, India

Presently at HIG-121, Lumbini Vihar, CSpur Area, Bhubaneswar, Odisha


Bioremediation of water is a sustainable measure for combating heavy metal and many other non-biodegradable, hazardous, toxic, etc pollutants. Cobalt is an essential element but toxic at high concentrations viz., 0.05 mg l−1 in drinking water. Cinamomum zylanicum bark (CzB, dalchini) is an ancient spice, aromatic and medicinal plant and finds wide application with recent focus on in health, food, agriculture, environment, etc. A substantial amount of bio-wastes are generated after CzB harvesting. However, a little is known for their utilization except oil extraction, bio-energy, green fertilizer, biocide, etc. Most of the symptoms of Co-toxicity and use of CzB for treatments appear to be closely matching. There may be a link between the Co and Cz compatibility of potential dimentions. The dry Cinamomum zylanicum leaf powder (CzLP) has pHpzc at about 6.3 with mostly carboxylate and minor amine/amide functional groups. So it can be used as an effective adsorbent for cations, anions, zwitterions, colloids, etc. The dilute acid and alkali leachates of CzLP have light green and dark tea coloration respectively. The biosorption of Co(II) (BSCo) on CzLP is studied for bioremediation of water and its potential agricultural and environmental applications. The pHi and pHt are influenced mainly by time, temperature, [CzLP] and [Co]. The effects of pH, temperature, time, etc are more pronounced than the effects of both [Co(II)] and [CzLP] on the BSCo indicating their interactions tending to saturation effect. Low pH, [CzLP] and temperature with high [Co] appears to be very effective for BSCo. The biosorption data is fitted to an empirical equation for ascertaining the parametric interactions. The CzLP surface adsorption/ co-precipitation processes of various Co(II) species appear to be the most plausible mechanism for BSCo. Only a part of the loaded Co on CzLP could be desorbed at pH 2 even in the presence of reductants indicating some bio-matrix bound Co(II) oxidation to Co(III). The BSCo capacity of CzLP is found to decrease with the increase of cycles Co–CzLP may be used for the bioremediation of Co-contaminated water and Co-harvesting/ concentrating from mine and electroplating industry effluents. Co-loaded CzLP waste may find application for biocide, nutrient reserve, fertilizer, soil emending, etc or be safe to dispose.

Keywords: Biosorption, cobalt, Cinamomum zylanicum leaf powder, biodesorption

Proximate composition and food value of ginger cultivars from different environmental conditions

Aradhana Das and *Enketeswara Subudhi

Centre for Biotechnology, Siksha O’ Anusandhan (Deemed To Be University), Bhubaneswar, Odisha, India

Aradhana Das

Centre of Biotechnology, Siksha ‘O’ Anusandhan (Deemed To Be University), Khandagiri, Bhubaneswar-751003, Odisha, India


Ginger (Zingiber officinale) is a well-known and widely used herb, which contains several remarkable bioactive constituents and possesses health-promoting properties. The moisture content, crude fibre, crude protein, fat, ash, and carbohydrate of ginger varieties at different environmental condition were studied to examine their nutritional value and its effect. The results obtained showed moisture contents (6.24 to 11.22%), indicating good shelf life characteristics. protein contents (17.46 to 20.69%) indicates a rich supplements to body functions for its growth, repair and maintenance of body protein. Crude fibre content (4.26 to 7.49%) indicates that these ginger varieties could efficiently be utilized to protect against obesity, cancer and weight reduction in obese individuals. Finally, the correlation analysis showed positive association of climatic condition on moisture content and crude fibre, which indicates the variation in percentage, due to climatic effect. Moreover, a strong correlation was seen between moisture content and crude fibre, which shows with increase of crude fibre there is decrease of carbohydrate content.  This study could be useful for selection of ginger varieties in commercial scale to maintain the nutritional value.



Kiran Prabha Nayaka, Antaryami Mishrab, Abanti Pradhanc, Aditya Kishore Dashc* and Subhashis Sarenb

a Department of Environmental Sciences, Sambalpur University, Burla, Odisha

b Department of Soil Science & Agricultural Chemistry, O.U.A.T., Bhubaneswar, Odisha

cBiofuel and Bioprocessing Research Center (BBRC), Institute of Technical Education and Research (ITER), Siksha


Present study assesses the soil fertility status of four villages of Sundargarh District, Odisha. Physico-chemical study of soil samples like colour, texture, pH, electrical conductivity, organic carbon, available nitrogen, phosphorus and potassium were measured.  Composite surface soil samples were collected up to the depth of 0-15 cm from three varying land types i.e. upland, medium land and lowland for analysis and compared with soil fertility rating. Soil samples collected from different sampling stations were mostly mixed yellow red soils. Soil texture of the four stations studied varies between loamy sand to loam. The clay percentage gradually increases from the upland to the low land soils in all the sampling sites. In general, the upland soils are found to be moderately acidic and the low land soils are found was slightly acidic to neutral. The electrical conductivity of surface soils of the four sampling sites varies between 0.08 to 0.65 dSm-1.  The high status of organic carbon in low lands of the sampling stations in comparison to upland and medium land is due to the accumulation of plant residues on the low lands that get transported from the uplands and get decomposed which contributes to higher organic carbon content. There is a positive correlation between organic carbon content and nitrogen content of those soils. The low concentration in available P2O5in the study area was because of the acidic nature of the soil. There is a higher potassium content in the low land soils of the four sites which may be due to the presence of more amount of clay. The present investigation showed the deficiency and sufficiency of available plant nutrients in soils which represent the different types of lands of North Western Plateau Agro Climatic Zone of  Odisha.

Keywords: Soil fertility, Soil physical parameters, NPK, Electrical conductivity, Organic carbon.

Solvent extraction of lanthanum (III) from acidic nitrate medium using Cyphos IL 104 and TOPO

*Niharbala Devi and Lala Behari Sukla

Biofuels and Bioprocessing Research Center, Institute of Technical Education and Research, Siksha ‘O’Anusandhan (Deemed to be University), Khandagiri Square, Bhubaneswar, OR, India


The red mud which poses a serious environmental issue for disposal contains a lot of metals including rare earth elements.  Many electronic wastes like batteries and lamps also contain good amount of rare earth elements, which is an alarming condition to the environment. Recover and reuse of the metal values is a challenge to separation scientist. In this context solvent extraction could be considered as it is a simpler, low cost technique. So, a systematic study of the extraction behavior of one of the rare earth element lanthanum from nitrate media using a bifunctional ionic liquid Cyphos IL 104 (Trihexyl(tetradecyl) phosphoniumbis(2,4,4-trimethylpentyl)phosphinate) and a neutral organophosphorous extractant TOPO(tri octyl phosphine oxide) was reported in this paper. Various parameters like acid concentration, extractant concentration, contact time, separation from other rare earth elements were investigated. The extraction percent increased with increase in aqueous phase pH and increasing the extractant concentration.  Three and fifteen minute contact times were required Cyphos IL 104 and TOPO, respectively to reach equilibrium. Separation studies from ternary mixture containing equimolar quantities of lanthanum, cerium and yttrium were investigated using both Cyphos IL 104 and TOPO diluted in toluene. Lanthanum could be completely separated from yttrium and cerium using TOPO.

Keywords: Lanthanum; Extraction; separation, Cyphos IL 104, TOPO

Isolation and characterization of organic solvent tolerant lipase producing Anoxybacillus sp ARS1 from Taptapani hot spring, India

Rajesh Kumar Sahoo and Enketeswara Subudhi*

Centre for Biotechnology, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, Odisha


There has been a great demand of organics solvent tolerant bacteria for industrial production of biochemical. In this study, we screened out a hexane enriched lipase producing isolate from Taptapani hot spring able to tolerate a range organic solvent and identified to be member of the genus Anoxybacillus. Anoxybacillus sp. ARS1 showed remarkable ability to tolerate solvents such as hexane, cyclohexane and octane at 50oC. The optimal temperature and pH for enzyme activity (35.78 IU/ml) was found to be at 50oC and pH 8.0 respectively. Anoxybacillus sp. ARS1 lipase preferred long carbon chain (4-nitrophenyl esters) and the maximum activity was observed with 4-nitrophenyl laurate as substrate. The presence of Na+, K+ Mg2+ and Ca2+ metal ions could enhance the enzyme activity. These biochemical features indicated that it is a potential biocatalyst producing bacteria for various industrial applications.

Keywords: Thermophiles, Organic solvent tolerance, Lipase, Hot spring, Anoxybacillus sp. ARS-1

Biotechnological applications of microalgae: A critical review

*Arun Kumar Pradhan a, Tanushree Mahapatra b, Madhusmita Hota b and Subhakanta Swain b

aCenter for Biotechnology, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, India

bUtkal University, Vanivihar, Bhubaneswar,751004


Unicellular microphyta present in both fresh water and marine ecosystem are called as microalgae. These microalgae may remain in individually or group form or in chain. In marine ecosystem microalgae acts as food for many animals such as some fishes, few mollusca and some crustacean species. Different microalgae contain different types of valuable products like pigments, antioxidants, fatty acids, anticancer chemicals etc. So these are having very high commercial values now a day. In this present study, we have discussed about many biotechnological aspects of microalgae. Pharmaceutical values of microalgae were discussed focusing their anticancer property and antioxidant property mainly. Microalgae and their products like protein, lipid and carbohydrates are also studied. We have also discussed about biodiesel producing microalgae.

Key Words: Microalgae; Biofertilizer; Anticaner; Biofuel; Cosmatics; Pigments

Salt stress response of diazotrophic cyanobacteria Nostoc sp. for their pigments and macromolecular contents

  1. Bhakta1*, J. Pradhan2, E. Sahu2, and A. K. Bastia2*
  2. Botanical Survey of India, Western Regional Centre, 7 Koregaon road,


  1. Department of Botany, North Orissa (Deemed to be University),



The present work is based on effect of salt stress on pigment composition and macromolecular contents of two cyanobacteria Nostoc linckia and Nostoc piscinale collected from local rice fields of Mayurbhanj district, Odisha. A varied response was observed in both the test organisms grown under different concentrations of NaCl. Both the cyanobacteria were well resistant to salt in the control and 10 mM NaCl concentration. The 10 mM NaCl increases growth, pigment composition and macromolecular contents of this test organism. Higher concentrations of 100mM NaCl inhibited growth, pigment composition and macromolecular contents of N. piscinale.  The organism could not survived at 200 mM of NaCl and found lethal. In N. linckia, growth, pigment contents and macromolecular synthesis was found maximum in control and decreased subsequently with increase in NaCl concentrations. 500 mM of Nacl was found the lethal to both the test organism. The experiment revealed that N. piscinale could be potent agriculturally important cyanobacteria for the saline soil type.

Key words: Salt stress, Pigments, Macromolecules, Diazotrophic cyanobacteria

Fungal Beneficiation of High Alumina and Silica Containing OMDC Iron Ore Tailing

*M. Pradhan1, M. Mishra2, C. C. Rath3, L. B. Sukla4

  1. G. department of Botany, North Orissa University, Takatpur, Baripada, Odisha, India,
  2. G. Dept. of Biosciences, College of Pharmaceutical Science, Mohuda, Berhampur, Odisha, India
  3. of Botany, College of Basic sciences and Humanities, OUAT, Bhubaneswar, Odisha, India
  4. Biofuels and Bioprocess Research Centre , Siksha ‘O’ Anusandhan (Deemed To Be University), Bhubaneswar, Odisha, India


Deposits of high grade ores are getting exhausted all over the world at an alarming rate due to rapid industrialization and heavy demand of metals. Low grade iron ore needs to be upgraded to increase the iron content and diminish the alumina, silica as high alumina, silica content in the iron ore affects coke rate, reducibility and productivity in blast furnace operation. Characterization study of OMDC iron ore tailing indicates that the major iron bearing minerals are hematite and Goethite. It contains 54.30% Fe, 6.83% alumina and 8.26 % Silica. Aspergillus niger, Aspergillusfumigatus, Penicilliumcitrinum, Penicilliumpurpurogenum and Aspergillusflavus  were screened for alumina and silica removal efficiency. Among the strains, Penicilliumcitrinum (MTCC NO.6999) was found to be most effective. It removed 55.34% alumina and 56.3% silica in 30 days at 10% pulp density, 35°C and at 150 rpm. The data was analysed statistically by one way ANOVA trough F-test.

Key words: Alumina; Silica, A. flavus; A. fumigatus; A. niger; P. citrinum; P. purpurogenum.

Use of Biobased Reagents for Mineral Processing: Need of the Time

Nilotpala Pradhan

Senior Scientist, Environment & Sustainability Department, CSIR-Institute of Minerals & Materials Technology, Bhubaneswar, Orissa,


Surfactants form an integral part of our everyday life with applications reaching far beyond our hygienic needs ranging from food to fuel additives all the way to compounds with antibiotic activities. Currently, surfactants are produced on a scale of almost 14 million tons worldwide. The majority of surfactants are still manufactured from fossil resources. There is a demand on replacement of petro-based surfactants by bio-based surfactants, which can be generated from renewable resources and are environment friendly. The focus hereby is on microbially and enzymatically produced biosurfactants obtained from natural renewable sources. Many chemical reagents like collectors, frother, activator, depressant, dispersant, modifier etc. are used in mineral process industries. Presently only few chemicals like pine oil and oleic acids are used as chemical reagents in mineral processing industry. Non availability of biosurfactants due to low production and high cost is main reason why they are not being used for mineral processing.

Algal Carotenoids as Future Antioxidant and Anticancerous Nutraceutical

 Debabrata Pradhan, Lala Behari Sukla

Lala Behari Sukla, Biofuels and Bioprocessing Research Center, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar-751030, India


Carotenoids are proven antioxidant. On oral consumption they are beneficial for protecting from diseases like cancer, obesity, and hypertension. Due to the health benefits, both natural and synthetic carotenoids are commercially produced as food, pharmaceuti­cal, and aquaculture. The market size of carotenoid products is at a rate of 2,200 tonnes per year and more than US$500 million year with the worldwide increasing demand at an average yearly rate of 2.2%. Furthermore, the demand for the natural carotenoids is higher than that of the synthetic form despite the high cost of the natural carotenoids. Natural carotenoids as nutraceutical are commercially produced from different carotenoid-rich fruits and vegetables. These fruits and vegetables are cultivated in the terrestrial plants. Since microalgae are more efficient than terrestrial plants in terms of biomass production, they are possibly rich candidates for becoming the major carotenoids producer if the economics of the production process works out favorably. Microalgae are microscopic photosynthetic organisms found in both marine and freshwater environments. They have high growth rate and can complete an entire growing cycle in every few days. The biomass doubling time of some microalgae are as short as 3.5 hours in the exponential growth phase. Food grade carotenoids, such as astaxanthin, lutein, and zeaxanthin, are generally produced in photobioreactors to avoid contamination during the biomass generation. Further, carotenoids can be extracted from the biomass of microalgae using state-of-art extraction process for different natural compounds. The carotenoid extract undergoes the geling process for the production of food grade carotenoids. Presently the status of research is inadequate for the production of food grade carotenoids from microalgae biomass. Therefore, pilot scale experimental study is necessary for the commercial production.

Keywords: Microalgae; Carotenoids; Extraction; Antioxidant properties; Nutraceutical



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